6R2N
Crystal structure of KlGlk1 glucokinase from Kluyveromyces lactis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.011, 122.771, 360.346 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.077 - 2.596 |
R-factor | 0.2051 |
Rwork | 0.204 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o8m |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.080 | 2.689 |
High resolution limit [Å] | 2.596 | 2.596 |
Rmerge | 0.046 | 0.482 |
Rmeas | 0.065 | 0.681 |
Rpim | 0.046 | 0.482 |
Number of reflections | 69999 | 6782 |
<I/σ(I)> | 13.6 | 1.7 |
Completeness [%] | 99.6 | 97.4 |
Redundancy | 2 | 2 |
CC(1/2) | 0.986 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.15 M KBr and 30 % PEG 2000 MME |