6R1D
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 7d, co-crystallized
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.643, 52.482, 59.242 |
| Unit cell angles | 90.00, 95.79, 90.00 |
Refinement procedure
| Resolution | 39.200 - 1.100 |
| R-factor | 0.12348 |
| Rwork | 0.122 |
| R-free | 0.14963 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v2y |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.449 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.160 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.053 | 0.400 |
| Number of reflections | 77977 | 12046 |
| <I/σ(I)> | 17.67 | 3.64 |
| Completeness [%] | 99.1 | 95.1 |
| Redundancy | 6.3 | 5.3 |
| CC(1/2) | 1.000 | 0.920 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 15 % PEG 6K, 5 % Glycerol |
