6R1A

Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 20b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.556, 59.309, 88.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 1.540
R-factor	0.18114
Rwork	0.180
R-free	0.20809
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4v2y
RMSD bond length	0.013
RMSD bond angle	1.895
Data reduction software	XDS
Data scaling software	XDS
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.180	1.640
High resolution limit [Å]	1.540	1.540
Rmerge	0.130	0.820
Number of reflections	44027	6798
<I/σ(I)>	10.28	1.71
Completeness [%]	99.5	96.7
Redundancy	12.6	12.1
CC(1/2)	1.000	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	0.4 m Ammonium phosphate