6R12
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 7b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.417, 58.795, 88.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.930 - 1.740 |
R-factor | 0.17703 |
Rwork | 0.174 |
R-free | 0.23398 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.026 |
RMSD bond angle | 2.259 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.930 | 1.840 |
High resolution limit [Å] | 1.730 | 1.730 |
Rmerge | 0.140 | 0.890 |
Number of reflections | 30720 | 54861 |
<I/σ(I)> | 10.55 | |
Completeness [%] | 98.8 | 92.4 |
Redundancy | 12.46 | 11.99 |
CC(1/2) | 1.000 | 0.860 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.4 M Ammonium phosphate |