6QZK
Structure of Clostridium butyricum Argonaute bound to a guide DNA (5' deoxycytidine) and a 19-mer target DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-16 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 181.450, 181.450, 142.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 76.606 - 3.548 |
R-factor | 0.2501 |
Rwork | 0.247 |
R-free | 0.27590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gq9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.606 | 3.675 |
High resolution limit [Å] | 3.548 | 3.548 |
Rmerge | 0.281 | 1.876 |
Rmeas | 0.284 | 1.907 |
Number of reflections | 17344 | 1696 |
<I/σ(I)> | 17.05 | 2.47 |
Completeness [%] | 99.9 | 99.94 |
Redundancy | 39 | 38 |
CC(1/2) | 0.999 | 0.861 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | NaForm, Nickel chloride |