6QWS
Crystal structure of the Ski2 RNA-helicase Brr2 from Chaetomium thermophilum in the apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 31 |
Unit cell lengths | 124.520, 124.520, 128.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.630 - 3.300 |
R-factor | 0.2096 |
Rwork | 0.206 |
R-free | 0.26150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m59 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.568 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.390 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmeas | 0.270 | |
Number of reflections | 33329 | 2454 |
<I/σ(I)> | 7.38 | 1.16 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 4.4 | |
CC(1/2) | 0.980 | 0.370 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Tris-HCl, PEG 3350, lithium sulfate |