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6QW2

The Transcriptional Regulator PrfA-A218G mutant from Listeria Monocytogenes

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE MASSIF-3
Synchrotron siteESRF
BeamlineMASSIF-3
Temperature [K]100
Detector technologyPIXEL
Collection date2018-02-19
DetectorDECTRIS EIGER X 4M
Wavelength(s)0.9680
Spacegroup nameP 21 21 21
Unit cell lengths47.837, 88.021, 116.386
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution48.543 - 2.600
R-factor0.1817
Rwork0.178
R-free0.25440
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5f1r
RMSD bond length0.002
RMSD bond angle0.445
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwarePHENIX ((1.12_2829: ???))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.6002.700
High resolution limit [Å]2.6002.600
Rmerge0.2311.860
Rpim0.0930.742
Number of reflections15707
<I/σ(I)>10.31.8
Completeness [%]99.999.9
Redundancy13.413.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.5291Droplets of 2 to 4 microl protein solution in 50 mM sodium phosphate pH 6.5 and 200 mM NaCl at 3 mg per ml were mixed with 2 microl reservoir solution consisting of 22-25% PEG 4000, 100 mM sodium citrate pH 5.5, and 17%

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