6QW2
The Transcriptional Regulator PrfA-A218G mutant from Listeria Monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-19 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9680 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.837, 88.021, 116.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.543 - 2.600 |
R-factor | 0.1817 |
Rwork | 0.178 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f1r |
RMSD bond length | 0.002 |
RMSD bond angle | 0.445 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.600 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.231 | 1.860 |
Rpim | 0.093 | 0.742 |
Number of reflections | 15707 | |
<I/σ(I)> | 10.3 | 1.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 13.4 | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Droplets of 2 to 4 microl protein solution in 50 mM sodium phosphate pH 6.5 and 200 mM NaCl at 3 mg per ml were mixed with 2 microl reservoir solution consisting of 22-25% PEG 4000, 100 mM sodium citrate pH 5.5, and 17% |