6QW2

The Transcriptional Regulator PrfA-A218G mutant from Listeria Monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9680
Spacegroup name	P 21 21 21
Unit cell lengths	47.837, 88.021, 116.386
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.543 - 2.600
R-factor	0.1817
Rwork	0.178
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f1r
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.231	1.860
Rpim	0.093	0.742
Number of reflections	15707
<I/σ(I)>	10.3	1.8
Completeness [%]	99.9	99.9
Redundancy	13.4	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291	Droplets of 2 to 4 microl protein solution in 50 mM sodium phosphate pH 6.5 and 200 mM NaCl at 3 mg per ml were mixed with 2 microl reservoir solution consisting of 22-25% PEG 4000, 100 mM sodium citrate pH 5.5, and 17%