6QW1
The Transcriptional Regulator PrfA-L140F mutant from Listeria Monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.634, 88.806, 115.439 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.396 - 1.844 |
R-factor | 0.1834 |
Rwork | 0.181 |
R-free | 0.24140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f1r |
RMSD bond length | 0.012 |
RMSD bond angle | 1.146 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 1.880 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.082 | 1.417 |
Rpim | 0.033 | 0.573 |
Number of reflections | 42882 | |
<I/σ(I)> | 17.7 | 1.9 |
Completeness [%] | 99.5 | 91.6 |
Redundancy | 13.4 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | Droplets of 2 to 4 microl protein solution in 50 mM sodium phosphate pH 6.5 and 200 mM NaCl at 3 mg per ml were mixed with 2 microl reservoir solution consisting of 22-25% PEG 4000, 100 mM sodium citrate pH 5.5, and 17% isopropanol. |