6QTV

Crystal structure of an Arabidopsis WD40 domain in complex with an atypical bHLH transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-13
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	48.896, 55.089, 103.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.580 - 1.310
R-factor	0.1353
Rwork	0.133
R-free	0.17610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5igo
RMSD bond length	0.007
RMSD bond angle	1.025
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.580	1.360
High resolution limit [Å]	1.310	1.310
Rmeas	0.035
Number of reflections	67056
<I/σ(I)>	16.09
Completeness [%]	99.8
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298.15	5 mg/mL of COP1 supplemented with 3 to 10 fold molar excess in peptide was mixed with two-fold (v/v) more mother liquor (1:2 ratio; protein:buffer) containing 1.25 M sodium malonate pH 7.5.