6QTM
Crystal structure of the Sir4 H-BRCT domain in complex with Ty5 pS1095 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.930, 58.930, 270.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 3.000 |
| R-factor | 0.2055 |
| Rwork | 0.201 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 10009 | |
| <I/σ(I)> | 7.7 | 1.1 |
| Completeness [%] | 97.1 | 95.8 |
| Redundancy | 4.2 | |
| CC(1/2) | 0.995 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 2.0 M ammonium sulfate 0.2 M sodium potassium tartrate 0.1 M tri-sodium citrate pH 5.6 |
