6QSZ
Crystal structure of the Sir4 H-BRCT domain in complex with Esc1 pS1450 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.060, 68.340, 77.810 |
| Unit cell angles | 72.61, 84.10, 87.41 |
Refinement procedure
| Resolution | 46.580 - 2.500 |
| R-factor | 0.1916 |
| Rwork | 0.189 |
| R-free | 0.23390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 42465 | |
| <I/σ(I)> | 7.69 | |
| Completeness [%] | 96.4 | 92.5 |
| Redundancy | 2 | 2 |
| CC(1/2) | 0.995 | 0.291 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 30% PEG 4000 0.2 M ammonium acetate 0.1 m sodium acetate pH 4.6 |
