6QSK
Crystal structure of a nucleotide sugar transporter with bound nucleotide monophosphate.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 83 |
Detector technology | PIXEL |
Collection date | 2017-09-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98010 |
Spacegroup name | P 1 |
Unit cell lengths | 45.705, 102.716, 181.253 |
Unit cell angles | 89.93, 90.08, 90.19 |
Refinement procedure
Resolution | 49.428 - 3.394 |
R-factor | 0.2559 |
Rwork | 0.253 |
R-free | 0.31960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5oge |
RMSD bond length | 0.012 |
RMSD bond angle | 1.381 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_3360: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.430 | 3.520 |
High resolution limit [Å] | 3.390 | 3.390 |
Rmerge | 0.100 | 0.680 |
Rmeas | 0.135 | 0.920 |
Rpim | 0.090 | 0.610 |
Number of reflections | 44054 | 4492 |
<I/σ(I)> | 5.4 | 1.2 |
Completeness [%] | 96.7 | 92.3 |
Redundancy | 2 | 2 |
CC(1/2) | 0.997 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5 | 277 | 26 - 30 % (v/v) PEG 400, 0.1 M sodium citrate pH 5.0 and 75 mM sodium chloride or sodium acetate. GMP was soaked in at 20mM overnight. |