6QNQ
70S ribosome initiation complex (IC) with experimentally assigned potassium ions
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-11-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.460, 452.180, 626.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 226.090 - 3.500 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.25130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4v6g |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 226.090 | 4.980 | 3.600 |
| High resolution limit [Å] | 3.500 | 4.850 | 3.500 |
| Rmerge | 0.285 | 0.568 | |
| Number of reflections | 719306 | 39133 | 53168 |
| <I/σ(I)> | 7.13 | 0.99 | 1.98 |
| Completeness [%] | 96.7 | 98 | 88.9 |
| Redundancy | 12.36 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-OAc, KSCN, PEG-20K, PEG-550mme |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-OAc, KSCN, PEG-20K, PEG-550mme |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-OAc, KSCN, PEG-20K, PEG-550mme |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-OAc, KSCN, PEG-20K, PEG-550mme |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Tris-OAc, KSCN, PEG-20K, PEG-550mme |
