6QL7
Structure of fatty acid synthase complex with bound gamma subunit from Saccharomyces cerevisiae at 4.6 angstrom
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 234.922, 430.307, 422.610 |
Unit cell angles | 90.00, 97.01, 90.00 |
Refinement procedure
Resolution | 192.500 - 4.600 |
R-factor | 0.2545 |
Rwork | 0.252 |
R-free | 0.30980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 1.226 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | MOLREP (11.6.04) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 192.500 | 4.700 |
High resolution limit [Å] | 4.600 | 4.600 |
Rpim | 0.088 | 0.829 |
Number of reflections | 458021 | 45686 |
<I/σ(I)> | 7.95 | 0.87 |
Completeness [%] | 99.5 | 99.71 |
Redundancy | 7.2 | 7.4 |
CC(1/2) | 0.998 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 0.1 M MES pH 6.2, 0.15-0.3 M ammonium sulphate, 7-8% (w/v) PEG 8000 |