6QK8
Crystal structure of yeast 14-3-3 protein (Bmh1) from Saccharomyces cerevisiae with the Nha1p (yeast Na+/H+ antiporter) 14-3-3 binding motif Ser481
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-11-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.850, 59.112, 66.700 |
| Unit cell angles | 95.90, 104.14, 91.19 |
Refinement procedure
| Resolution | 41.141 - 2.917 |
| R-factor | 0.2204 |
| Rwork | 0.218 |
| R-free | 0.27080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5n6n |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.141 | 3.022 |
| High resolution limit [Å] | 2.917 | 2.917 |
| Rmeas | 0.167 | 0.617 |
| Number of reflections | 16913 | 1720 |
| <I/σ(I)> | 7.23 | 2.15 |
| Completeness [%] | 92.8 | 94.51 |
| Redundancy | 2.98 | 4.81 |
| CC(1/2) | 0.986 | 0.723 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293.15 | KBr, PEG 2K MME |
