6QJH
Cryo-EM structure of heparin-induced 2N4R tau snake filaments
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2 |
Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.3 |
Number of Particle | 303754 |
Software | RELION,REFMAC,PHENIX,EPU,CTFFIND,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |