6QID

Crystal structure of DEAH-box ATPase Prp43-S387A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-18
Detector	DECTRIS PILATUS3 R 200K-A
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	88.820, 105.450, 119.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.614 - 2.204
R-factor	0.1849
Rwork	0.184
R-free	0.20810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ltj
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.614	50.000	2.300
High resolution limit [Å]	2.200	35.614	2.200
Rmerge	0.066		0.566
Rmeas	0.071		0.646
Number of reflections	57004		6587
<I/σ(I)>	20.81		2.51
Completeness [%]	99.1		93
Redundancy	7.474		4.198
CC(1/2)	0.999		0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM HEPES pH 7, 3% PEG4000, 35% MPD