6QF9
Structure of an anti-Mcl1 scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.165, 62.749, 70.557 |
| Unit cell angles | 90.00, 105.52, 90.00 |
Refinement procedure
| Resolution | 52.190 - 1.430 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.21600 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.749 | 1.266 |
| High resolution limit [Å] | 1.244 | 1.244 |
| Number of reflections | 97755 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 93.0 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG4k, 10% 2-propanol, 0.1M HEPES, pH 7.5 |
