6QF6
Structure of an E.coli expressed anti-Mcl1 scFv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-24 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 180.175, 180.175, 88.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 127.400 - 2.590 |
Rwork | 0.181 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6qb3 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 127.403 | 3.010 |
High resolution limit [Å] | 2.590 | 2.590 |
Number of reflections | 23417 | 26817 |
<I/σ(I)> | 5.75 | |
Completeness [%] | 91.3 | |
Redundancy | 12.92 | |
CC(1/2) | 0.978 | 0.369 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.4 M Ammonium Sulphate, 25% PEG 3350, 0.1 M BIS-TRIS pH 5.9 |