6QF5

X-Ray structure of human Aquaporin 2 crystallized on a silicon chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE MFX
Synchrotron site	SLAC LCLS
Beamline	MFX
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-11-13
Detector	CS-PAD XPP
Wavelength(s)	1.3670
Spacegroup name	P 42
Unit cell lengths	122.200, 122.200, 94.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.892 - 3.700
R-factor	0.2873
Rwork	0.282
R-free	0.33570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nef
RMSD bond length	0.004
RMSD bond angle	0.761
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.10.1-2155_9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.380	3.760
High resolution limit [Å]	3.700	3.700
Number of reflections	14866	1462
<I/σ(I)>	2.32	0.92
Completeness [%]	100.0	99.93
Redundancy	28.1	17.7
CC(1/2)	0.783	0.105

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	0.1M Tris pH 8.5, 0.1M sodium chloride, 0.1M magnesium chloride, 22-25% PEG 400, cadmium chloride