6QD5

X-ray Structure of the Human Urea Channel SLC14A1/UT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 63
Unit cell lengths	112.331, 112.331, 87.940
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.335 - 2.398
R-factor	0.1777
Rwork	0.175
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ezc
RMSD bond length	0.009
RMSD bond angle	1.046
Data reduction software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.335	2.484
High resolution limit [Å]	2.398	2.398
Rpim	0.047	0.614
Number of reflections	24788	2434
<I/σ(I)>	9.02	1.29
Completeness [%]	99.8	98.66
Redundancy	2	2
CC(1/2)	0.996	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	36% PEG400 -- 0.1M tris pH 8.5 -- 0.05M lithium sulfate -- 0.05M sodium sulfate