6QB6
Mcl1 in complex with a Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.050, 42.460, 106.230 |
| Unit cell angles | 90.00, 113.19, 90.00 |
Refinement procedure
| Resolution | 47.710 - 2.240 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.100 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Number of reflections | 29461 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 54.5 mM PCTP4 45.5 mM PCTP10 15 %w/v PEG-2000 MME |
