6QB5
Crystal structure of the N-terminal region of human cohesin subunit STAG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.611, 124.985, 231.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.002 - 2.020 |
| R-factor | 0.2197 |
| Rwork | 0.218 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pk7 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.341 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.000 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.081 | 0.969 |
| Rpim | 0.052 | 0.583 |
| Number of reflections | 125560 | 9012 |
| <I/σ(I)> | 9.8 | 1.1 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 6 | 4.4 |
| CC(1/2) | 0.998 | 0.664 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Na/K Phosphate pH 6.0, 0.2 M NaCl, 34% PEG200 |
