6QB3
Apo Mcl1 in a complex with a scFv
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.080, 40.380, 75.420 |
| Unit cell angles | 90.00, 110.50, 90.00 |
Refinement procedure
| Resolution | 38.660 - 1.900 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.850 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 32161 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 99.4 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PCPT 0.1M pH 7.5, PEG 3350 15%w/v, MgCl2 0.1M |
