6Q8Q

Bovine Insulin under 2 kbar of argon

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.9997
Spacegroup name	I 21 3
Unit cell lengths	77.559, 77.559, 77.559
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.810 - 2.000
R-factor	0.18262
Rwork	0.182
R-free	0.19900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3bn3
RMSD bond length	0.010
RMSD bond angle	1.774
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	FFT
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.302
Rmeas	0.081	0.218
Rpim	0.016	0.087
Number of reflections	5259	673
<I/σ(I)>	31.3	7.8
Completeness [%]	97.8	85.4
Redundancy	30.4	11.6
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	PROTEIN DROP: 10 MG/ML. RESERVOIR: 1.2 M TMAO + 0.1 M SODIUM MALATE, PH 5.5