6Q5Y
Crystal structure of the SPOC domain of human PHF3 in complex with RNA polymerase II CTD diheptapeptide phosphorylated on Ser2Ser5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0723 |
Spacegroup name | P 32 |
Unit cell lengths | 67.832, 67.832, 178.118 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.760 - 2.850 |
R-factor | 0.19394 |
Rwork | 0.192 |
R-free | 0.22689 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6q2v |
RMSD bond length | 0.005 |
RMSD bond angle | 1.385 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.530 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.157 | 1.432 |
Rmeas | 0.183 | 1.662 |
Rpim | 0.124 | 0.819 |
Number of reflections | 21184 | 3104 |
<I/σ(I)> | 6.6 | |
Completeness [%] | 98.8 | 99.5 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.03 M MgCl2, 0.03 M CaCl2, 0.1 M imidazole-MES pH 6.5, 20% glycerol and 10% PEG4000 |