6Q5A
Crystal structure of Cryptosporidium hominis CPSF3 in the apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 90.360, 90.360, 238.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.490 - 1.550 |
R-factor | 0.13969 |
Rwork | 0.138 |
R-free | 0.17931 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6q55 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.293 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.660 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmeas | 0.070 | 0.900 |
Number of reflections | 82588 | 14867 |
<I/σ(I)> | 15.7 | 2.6 |
Completeness [%] | 98.0 | 96.8 |
Redundancy | 10.1 | 9.3 |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | 17% (v/v) Poly Ethylene Glycol 4000, 10% (v/v) Isopropanol, 100 mM HEPES pH 7.5 |