6Q45
F1-ATPase from Fusobacterium nucleatum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.954 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 111.940, 200.209, 201.725 |
Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
Resolution | 49.340 - 3.600 |
R-factor | 0.2395 |
Rwork | 0.237 |
R-free | 0.28010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ik2 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.759 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.290 | 49.290 | 3.660 |
High resolution limit [Å] | 3.600 | 19.730 | 3.600 |
Rmerge | 0.209 | 0.109 | 1.055 |
Rmeas | 0.229 | 0.117 | 1.200 |
Rpim | 0.090 | 0.043 | 0.555 |
Total number of observations | 592580 | 3583 | 20356 |
Number of reflections | 97166 | 570 | 4646 |
<I/σ(I)> | 6.4 | 15.5 | 1.5 |
Completeness [%] | 97.0 | 88.6 | 94.6 |
Redundancy | 6.1 | 6.3 | 4.4 |
CC(1/2) | 0.983 | 0.983 | 0.551 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1 ul protein to 0.8ul 100 mM sodium citrate, pH 6.0, 100 mM magnesium acetate and 15.5% [w/v] polyethylene glycol 5000 monomethyl ether and 0.2 ul low melting point agarose |