6Q3U
Gly52Ala mutant of arginine-bound ArgBP from T. maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 92.572, 52.238, 59.889 |
| Unit cell angles | 90.00, 117.39, 90.00 |
Refinement procedure
| Resolution | 14.800 - 1.640 |
| R-factor | 0.16466 |
| Rwork | 0.163 |
| R-free | 0.18923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ggv |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.234 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.700 |
| High resolution limit [Å] | 1.640 | 1.640 |
| Rmerge | 0.064 | |
| Number of reflections | 30030 | |
| <I/σ(I)> | 30.3 | |
| Completeness [%] | 96.1 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.0 M ammonium sulfate, 0.1 M TRIS hydrochloride buffer pH 8.5 |
