6Q2V
Crystal structure of SPOC domain of human PHD-finger protein 3 (PHF3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97863 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.745, 69.306, 127.215 |
| Unit cell angles | 90.00, 100.09, 90.00 |
Refinement procedure
| Resolution | 48.779 - 2.594 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (dev_3150) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.780 | 2.710 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.105 | 0.867 |
| Rmeas | 0.126 | 1.170 |
| Rpim | 0.069 | 0.790 |
| Number of reflections | 32812 | 2252 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 87.8 | 50.3 |
| Redundancy | 6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | containing 0.2 M MgCl2, 0.1M Bis-Tris pH 5.5, 25%PEG 3350 |
