6Q2Q
Crystal structure of mouse viperin bound to uridine triphosphate and S-adenosylhomocysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.455, 141.265, 143.488 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.303 - 1.892 |
R-factor | 0.1477 |
Rwork | 0.146 |
R-free | 0.18230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vsl |
RMSD bond length | 0.008 |
RMSD bond angle | 0.953 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.892 | 1.892 |
Rmerge | 0.146 | 0.566 |
Number of reflections | 58202 | 5740 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 96.6 | |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM Bis-tris propane, pH 6.2-6.8, 200 mM NaCl, 19-22% (w/v) polyethylene glycol 4000 or 6000, 6 mM S-adenosylhomocysteine, and 10 mM UTP |