6Q2D
Crystal structure of Methanobrevibacter smithii Dph2 in complex with Methanobrevibacter smithii elongation factor 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.899, 322.979, 172.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.356 - 3.450 |
R-factor | 0.226 |
Rwork | 0.224 |
R-free | 0.26650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Methanobrevibacter smithii Dph2 Pyrococcus horikoshii EF-2 (5H7J) |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 3.640 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.075 | |
Number of reflections | 37395 | 5369 |
<I/σ(I)> | 8.7 | |
Completeness [%] | 99.7 | |
Redundancy | 4.1 | |
CC(1/2) | 0.998 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | The reservoir solution contained 100 mM Tris, pH 7.2, 200 mM MgSO4, and 21 % (w/v) PEG400; NaCl was added to the reservoir to a final concentration of 300 mM after forming the drop |