6Q1U
Structure of plasmin and peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-12-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.953732013702 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.228, 80.293, 83.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.691 - 2.347 |
R-factor | 0.2352 |
Rwork | 0.233 |
R-free | 0.28740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d3x |
RMSD bond length | 0.004 |
RMSD bond angle | 0.702 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.691 | 2.431 |
High resolution limit [Å] | 2.347 | 2.347 |
Rmerge | 0.189 | 0.714 |
Rmeas | 0.214 | 0.814 |
Rpim | 0.097 | 0.379 |
Number of reflections | 22667 | 2197 |
<I/σ(I)> | 5 | 1.8 |
Completeness [%] | 98.9 | 98.03 |
Redundancy | 4.2 | |
CC(1/2) | 0.991 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM Sodium Citrate, 100 mM MgCl2, 18% PEG 4000 |