6Q1U

Structure of plasmin and peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.953732013702
Spacegroup name	P 21 21 21
Unit cell lengths	79.228, 80.293, 83.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.691 - 2.347
R-factor	0.2352
Rwork	0.233
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d3x
RMSD bond length	0.004
RMSD bond angle	0.702
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.691	2.431
High resolution limit [Å]	2.347	2.347
Rmerge	0.189	0.714
Rmeas	0.214	0.814
Rpim	0.097	0.379
Number of reflections	22667	2197
<I/σ(I)>	5	1.8
Completeness [%]	98.9	98.03
Redundancy	4.2
CC(1/2)	0.991	0.819

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM Sodium Citrate, 100 mM MgCl2, 18% PEG 4000