6Q0W

Structure of DDB1-DDA1-DCAF15 complex bound to Indisulam and RBM39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	93.973, 81.799, 260.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 2.900
R-factor	0.239
Rwork	0.238
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q0r
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.990	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.160	2.600
Number of reflections	45547	4294
<I/σ(I)>	7	0.7
Completeness [%]	99.0	97.3
Redundancy	6.7
CC(1/2)	0.990	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	20% PEG 4000