6Q0S

Crystal Structure of RSV strain B18537 Prefusion-stabilized glycoprotein F Variant DS-Cav1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 3 2
Unit cell lengths	167.874, 167.874, 167.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.270 - 1.940
R-factor	0.189
Rwork	0.187
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mmu
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.940	1.940
Rmerge	0.100	0.788
Number of reflections	57616	3616
<I/σ(I)>	11.96	1.12
Completeness [%]	95.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	1.2 M ammonium sulfate, 0.1 M lithium sulfate, 0.1 M sodium acetate pH 5.5