6PZR
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 complexed with Resminostat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.883, 97.364, 97.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.940 - 2.300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eem |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.940 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.361 | 1.298 |
Rpim | 0.154 | 0.547 |
Number of reflections | 32376 | 3216 |
<I/σ(I)> | 3.4 | 1.4 |
Completeness [%] | 98.6 | |
Redundancy | 6.4 | |
CC(1/2) | 0.977 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10 mg/mL zCD2 protein, 2 mM Resminostat inhibitor, 0.2 M potassium iodide, 20% w/v PEG3350, 1:1 ratio protein to precipitant |