6PZQ
Structure of the human respiratory syncytial virus M2-1 protein in complex with a short positive-sense gene-end RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-04-15 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 94.750, 94.750, 199.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.517 - 2.703 |
R-factor | 0.2281 |
Rwork | 0.225 |
R-free | 0.28760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c3b |
RMSD bond length | 0.017 |
RMSD bond angle | 1.470 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.750 | 2.850 |
High resolution limit [Å] | 2.703 | 2.703 |
Rmerge | 0.127 | |
Rmeas | 0.132 | |
Rpim | 0.027 | 0.390 |
Number of reflections | 25701 | 3673 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 24.3 | 26 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 25% w/v polyethylene glycol 3,350 |