6PYP
Binary Complex of Human Glycerol 3-Phosphate Dehydrogenase, R269A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.330, 76.560, 64.610 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 42.000 - 1.950 |
| Rwork | 0.167 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Starting model was an in-house model of open conformation |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.074 | 0.213 |
| Rmeas | 0.103 | 0.295 |
| Rpim | 0.071 | 0.202 |
| Number of reflections | 44671 | 3113 |
| <I/σ(I)> | 8.6 | 4.5 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.1 | |
| CC(1/2) | 0.993 | 0.919 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 23% PEG 20000 175 mM KCl 0.1M Bis-Tris propane |
