6PY8
Crystal structure of the RBPJ-NOTCH1-NRARP ternary complex bound to DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.880, 103.650, 301.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.460 - 3.750 |
R-factor | 0.27 |
Rwork | 0.267 |
R-free | 0.31500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f8x |
RMSD bond length | 0.002 |
RMSD bond angle | 0.573 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.460 | 3.888 |
High resolution limit [Å] | 3.754 | 3.754 |
Number of reflections | 26271 | 2504 |
<I/σ(I)> | 7.39 | |
Completeness [%] | 99.2 | |
Redundancy | 4.4 | |
CC(1/2) | 0.998 | 0.689 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 50 mM Hepes pH 6.8, 200 mM Sodium Fluoride, 18% PEG 3350 |