6PXL
3.74 Angstroms resolution structure of HlsU with an axial-channel plug
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-06 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 414.594, 92.331, 200.849 |
Unit cell angles | 90.00, 114.37, 90.00 |
Refinement procedure
Resolution | 49.560 - 3.741 |
R-factor | 0.2225 |
Rwork | 0.220 |
R-free | 0.26510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1do0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.810 |
High resolution limit [Å] | 3.740 | 3.740 |
Rmerge | 0.121 | 0.562 |
Rmeas | 0.140 | 0.694 |
Rpim | 0.068 | 0.397 |
Number of reflections | 68296 | 68296 |
<I/σ(I)> | 10.3 | 1.17 |
Completeness [%] | 94.6 | |
Redundancy | 3.8 | |
CC(1/2) | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M ammonium sulfate, 18% PEG-3350, 0.1 M TRIS pH 7.5 |