6PXC

N-Terminal SH2 domain of the p120RasGAP bound to a p190RhoGAP phosphotyrosine peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	I 2 2 2
Unit cell lengths	44.190, 64.827, 87.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.620 - 1.600
R-factor	0.1858
Rwork	0.184
R-free	0.20970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k44
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.158	0.135	1.110
Rmeas	0.163	0.139	1.182
Rpim	0.039	0.033	0.398
Total number of observations	265969
Number of reflections	16868	1800	1652
<I/σ(I)>	11.3
Completeness [%]	100.0	100	99.9
Redundancy	15.8	17.3	8.6
CC(1/2)		0.994	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	1.8M Sodium Malonate 0.1M Bis Tris 6.5 2% PEG MME 550