6PWZ
Crystal structure of human uridine-cytidine kinase 2 complexed with 2'-azidocytidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.730, 84.740, 153.579 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 83.200 - 3.000 |
| R-factor | 0.1986 |
| Rwork | 0.197 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6n55 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.200 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.057 | 0.432 |
| Rmeas | 0.081 | 0.611 |
| Rpim | 0.057 | 0.432 |
| Number of reflections | 48286 | 4783 |
| <I/σ(I)> | 6.5 | 1.65 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 6.1 | 2 |
| CC(1/2) | 0.996 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tris, pH 6.5, 25% pentaerythritol ethoxylate (15/4 EO/OH) |
