6PV4
Structure of CpGH84A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-14 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.090, 92.110, 103.080 |
| Unit cell angles | 95.74, 110.52, 119.71 |
Refinement procedure
| Resolution | 75.450 - 2.200 |
| R-factor | 0.2042 |
| Rwork | 0.202 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.167 | 0.386 |
| Rpim | 0.113 | 0.262 |
| Number of reflections | 129154 | 18702 |
| <I/σ(I)> | 7.7 | 5.6 |
| Completeness [%] | 98.1 | 97.3 |
| Redundancy | 2.7 | 2.7 |
| CC(1/2) | 0.929 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 14% PEG3350, 0.15 M CaCl2 and 20 mM octyl-beta-D-glucopyranoside |
