6PUQ

1.56 A crystal structure of flavodoxin-like domain of Schizosaccharomyces japonicus putative tRNAPhe 4-demethylwyosine synthase Tyw1 in complex with FMN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	28.298, 73.087, 42.518
Unit cell angles	90.00, 91.86, 90.00

Refinement procedure

Resolution	42.496 - 1.560
R-factor	0.1992
Rwork	0.196
R-free	0.22710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	DIALS
Data scaling software	Aimless (0.5.27)
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.090	1.600
High resolution limit [Å]	1.560	1.560
Rmerge	0.075	0.978
Rmeas	0.082	1.162
Rpim	0.033	0.612
Number of reflections	41931	743
<I/σ(I)>	11.2
Completeness [%]	87.9	41.5
Redundancy	5.7	3.4
CC(1/2)	0.996	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	288	200 mM sodium acetate trihydrate, 30% PEG 4000, 100 mM Tris- HCl pH 8.5