6PTH
Crystal structure of a DnaN sliding clamp (DNA polymerase III subunit beta) from Pseudomonas aeruginosa bound to griselimycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-07-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 139.650, 139.650, 173.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.711 - 3.050 |
R-factor | 0.1934 |
Rwork | 0.190 |
R-free | 0.22360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tsz |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.16_3546: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.711 | 45.711 | 3.130 |
High resolution limit [Å] | 3.050 | 13.640 | 3.050 |
Rmerge | 0.061 | 0.026 | 0.608 |
Rmeas | 0.066 | 0.029 | 0.648 |
Total number of observations | 157945 | ||
Number of reflections | 19666 | 266 | 1415 |
<I/σ(I)> | 21.4 | 43.72 | 2.87 |
Completeness [%] | 99.8 | 91.7 | 100 |
Redundancy | 8.031 | 5.741 | 8.334 |
CC(1/2) | 0.999 | 0.999 | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | PsaeA.17987.a.EN11.PD38369 at 20.74 mg/mL with 0.8 mM griselimycin against MCSG screen condition A5 optimization screening 0.1 M sodium acetate pH 4.2, 1.4 M ammonium sulfate, 0.2 M sodium chloride, supplemented with 25% ethylene glycol as cryo-protectant; crystal tracking ID 310486b9, unique puck ID bbz4-5 |