6PPQ
Structure of S. pombe Lsm1-7 with RNA, polyuridine with 3' adenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976177 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 68.857, 68.857, 296.297 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.500 - 1.810 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.800 | 1.880 |
High resolution limit [Å] | 1.810 | 1.810 |
Number of reflections | 75844 | 4347 |
<I/σ(I)> | 15.4 | |
Completeness [%] | 100.0 | |
Redundancy | 31.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 289 | 20 mM sodium formate, 20 mM ammonium acetate, 20 mM trisodium citrate, 20 mM sodium potassium tartrate, 20 mM sodium oxamate, 100 mM sodium HEPES base, 100 mM MOPS acid, 10 % PEG 8,000, 20 % ethylene glycol |