6PMB
TRK-A IN COMPLEX WITH LIGAND 1a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 52.109, 52.109, 226.747 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.170 - 2.810 |
R-factor | 0.2193 |
Rwork | 0.214 |
R-free | 0.28210 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.011 |
RMSD bond angle | 1.756 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.580 | 3.060 |
High resolution limit [Å] | 2.810 | 2.810 |
Number of reflections | 8869 | 1919 |
<I/σ(I)> | 19.81 | |
Completeness [%] | 99.1 | 96.4 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.10M KH2PO4/ 0.10M NaH2PO4/ 0.1M MES/NaOH pH = 6.00 and 1.9M NaCl (cryo: 25% glycerol in reservoir), or 18% (w/v) PEG 3350, 0.2M CaCl2, 0.10M, MES pH = 6.50 (cryo: direct) |