6PLM
Legionella pneumophila SidJ/ Calmodulin 2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.351, 103.792, 110.194 |
| Unit cell angles | 90.00, 104.69, 90.00 |
Refinement procedure
| Resolution | 29.330 - 2.592 |
| Rwork | 0.176 |
| R-free | 0.24055 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.817 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 2.660 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.091 | 0.797 |
| Number of reflections | 69809 | 3687 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 97.7 | |
| Redundancy | 6.908 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris pH 9.2, 0.2 M Sodium Iodide , 15% PEG 3350, 1 mM Calcium Chloride, 1 mM ATP |
