6PLM

Legionella pneumophila SidJ/ Calmodulin 2 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	105.351, 103.792, 110.194
Unit cell angles	90.00, 104.69, 90.00

Refinement procedure

Resolution	29.330 - 2.592
Rwork	0.176
R-free	0.24055
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.817
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	2.660
High resolution limit [Å]	2.590	2.590
Rmerge	0.091	0.797
Number of reflections	69809	3687
<I/σ(I)>	13.2
Completeness [%]	97.7
Redundancy	6.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris pH 9.2, 0.2 M Sodium Iodide , 15% PEG 3350, 1 mM Calcium Chloride, 1 mM ATP