6PLC

Structure of human DNA polymerase eta complexed with 8OA in the template base paired with incoming non-hydrolyzable GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9765
Spacegroup name	P 61
Unit cell lengths	98.447, 98.447, 81.856
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.1834
Rwork	0.179
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.011
RMSD bond angle	1.659
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.300	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.067	0.370
Number of reflections	15731	788
<I/σ(I)>	20.9
Completeness [%]	100.0
Redundancy	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.1M MES (pH 5.5), 5 mM MgCl2, and 15 to 20 % PEG 2K-MME