6PK8

Antibody scFv-M204 dimeric state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	60.600, 105.260, 176.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.410 - 2.910
R-factor	0.1909
Rwork	0.186
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gs3
RMSD bond length	0.009
RMSD bond angle	1.616
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.410	90.410	2.990
High resolution limit [Å]	2.910	13.020	2.910
Rmerge	0.171	0.043	0.975
Rmeas	0.198	0.052	1.144
Number of reflections	25041	331	1651
<I/σ(I)>	6.67	18.04	1.17
Completeness [%]	98.1	95.9	89.8
Redundancy	3.73	3.266	3.475
CC(1/2)	0.988	0.998	0.481

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.15 M Ammonium sulfate, 0.1 M MES pH 6.0, 15% PEG 4000